Phytohormone targeting in plant tissues

Methods Homogenization and extraction with organic solvents was done in one microcentrifuge tube and accelerated by crushing the plant material in a vibration mill. The extracts from minute amounts of fresh plant material were immediately purified using a solid-phase extraction (C18, C8, OasisTM HLB cartridges)in combination with ion-exchange and/or immunoaffinity purification. A fast chromatography technique, the ultra performance liquid chromatography (Acquity UPLC,Waters) was coupled to triple quadrupole mass spectrometer (Xevo TQ MS, Waters) equipped with an electrospray interface (ESI) and the unique performance of collision cell – ScanWave. The mass spectrometric conditions were optimized for each analyte and quantification was obtained by multiple reaction monitoring (MRM) of precursors and the appropriate product ions.